Blast Tube-Compatible Neuronal Cell Petri Dish Compartment

This project presents a neuronal cell blast injury device. This device will be used as a container for petri dishes holding neurons, and will be placed in a blast tube (shown in Figure 1 below) for experiments studying the effect of mechanical and thermal stress on neuron survival.

Figure 1 – (a) Blast Tube Assembly 2D Sketch; and (b) Blast Tube Image

The design must account for the following:

• Area of the device is less than or equal 20% of the blast tube cross sectional area.
• Quick insertion of the PDMS substrate inside the apparatus (no bolts).
• Elimination of all air bubbles.
• Mounting inside the blast tube.
• Fitting the PDMS substrate inside a petri dish.
• Biocompatible and autoclavable material. 
• Pressure measurement.

With those criterion in mind, the device’s main body is composed of PTFE, the lid is Acrylic (for visibility), and the body holders are of aluminum. Figures 2 – 10 below show renders and 2D sketches of the build.

Figure 2 – Compartment Render Images

Figure 3 – (a) PDMS Plate 2D Sketch; and (b) PDMS Mold 2D Sketch

Figure 4 – (a) Acrylic Lid 2D Sketch; and (b) PTFE Main Body 2D Sketch

Figure 5 – Holding Body 2D Sketch

Figure 6 – Main Body, Lid, and Holding Body Detailed 2D Sketch

Figure 7- (a) Separated Assembly; and (b) Combined Assembly